教育经历:

1994 - 1998　　　　本科, 遗传学, 北京大学
1998 - 2003　　　　博士, 细胞生物学, 北京大学

工作经历:

2004 - 2010　　　　 英国剑桥大学Gurdon研究所，博士后
2010 - 现在　　　　 研究员, beat365官方网站生物医学前沿创新中心（BIOPIC）
2015 - 现在　　　　 研究员, 北大-清华生命科学联合中心
2017 - 现在　　　　 教授, beat365官方网站生物医学前沿创新中心（BIOPIC）

荣誉奖励:

2014年度中国科学十大进展（“利用极体高通量测序结果精确推演出母源基因组信息”）
2015年度中国科学十大进展（“揭示人类原始生殖细胞基因表达与表观遗传调控特征”）
2015年度中国生命科学领域十大进展
2019年度中国生命科学领域十大进展

杂志编辑:

2020-至今 《Cell Stem Cell》编委（Editorial Board Member）； 2016-至今 《Open Biology》编委（Editorial Board Member）； 2015-至今 《Genome Biology》编委（Editorial Board Member）； 2014-2017 《Science Bulletin》编委（Editorial Board Member）；

      具有自我更新能力和分化潜能的干细胞是人类胚胎发育过程中以及成年个体生命活动中的关键类型的细胞，对各种干细胞进行深入研究是理解人类发育、生长机制的关键，也是将干细胞应用于临床再生医学、治疗人类疾病的前提。本实验室主要围绕人类早期胚胎发育研究多能干细胞的自我更新和多能性调控的分子机理，特别是表观遗传调控机理，以及相关的生殖系细胞发育过程中的表观遗传学重编程机理。利用我们发展的单细胞功能基因组学高通量测序技术体系（单细胞转录组测序技术、单细胞 DNA甲基化组测序技术、单细胞染色质状态组测序技术、单细胞多组学平行测序技术等），以及基因编辑技术、哺乳动物胚胎显微操作技术、器官小体（organoid）培养技术、以及人类胚胎干细胞体外定向分化等技术在单细胞和单碱基的极限分辨率下深入研究人类生殖系细胞发育、以及多能性干细胞分化过程中基因表达网络的表观遗传学调控机理。并在此基础上深入探索生殖细胞发育异常相关的不孕不育等疾病的分子机理和潜在的治疗策略。

1. Li L, Li L, Li Q, Liu X, Ma X, Yong J, Gao S, Wu X, Wei Y, Wang X, Wang W, Li R, Yan J, Zhu X, Wen L, Qiao J, Yan L*, Tang Fuchou*. Dissecting the epigenomic dynamics of human fetal germ cell development at single-cell resolution. Cell Research, doi.org/10.1038/s41422-020-00401-9 (2020) (*: Co-corresponding authors).
2. Fan X, Fu Y, Zhou X, Sun L, Yang M, Wang M, Chen R, Wu Q, Yong J, Dong J, Wen L, Qiao J*, Wang X*, Tang Fuchou*. Single-cell transcriptome analysis reveals cell lineage specification in temporal-spatial patterns in human cortical development. Science Advances, 6: eaaz2978 (2020) (*: Co-corresponding authors).
3. Gao Y, Li L, Yuan P, Zhai F, Ren Y, Yan L, Li R, Lian Y, Zhu X, Wu X, Kee K, Wen L, Qiao J*, Tang Fuchou*. 5-Formylcytosine landscapes of human preimplantation embryos at single-cell resolution. PLOS Biology, doi.org/10.1371/journal.pbio.3000799 (2020) (*: Co-corresponding authors).
4. Yu K, Hu Y, Wu F, Guo Q, Qian Z, Hu W, Chen J, Wang K, Fan X, Wu X, Rasko J, Fan X, Iavarone A*, Jiang T*, Tang Fuchou*, Su X*. Surveying brain tumor heterogeneity by single-cell RNA sequencing of multi-sector biopsies. National Science Review, doi.org/10.1093/nsr/nwaa099 (2020) (*: Co-corresponding authors).
5. Li J, Zheng Y, Yan P, Song M, Wang S, Sun L, Liu Z, Ma S, Izpisua Belmonte JC, Chan P, Zhou Q, Zhang W*, Liu G*, Tang Fuchou*, Qu J*. A Single-cell Transcriptomic Atlas of Primate Pancreatic Islet Aging. National Science Review, doi.org/10.1093/nsr/nwaa127 (2020) (*: Co-corresponding authors).
6. Zhang W, Zhang S, Yan P, Ren J, Song M, Li J, Lei J, Pan H, Wang S, Ma X, Ma S, Li H, Sun F, Wan H, Li W, Chan P, Zhou Q, Liu GH*, Tang Fuchou*, Qu J*. A single-cell transcriptomic landscape of primate arterial aging. Nature Communications, 11: 2202 (2020) (*: Co-corresponding authors).
7. Hou S, Li Z, Zheng X, Gao Y, Dong J, Ni Y, Wang X, Li Y, Ding X, Chang Z, Li S, Hu Y, Fan X, Hou Y, Wen L, Liu B*, Tang Fuchou*, Lan Y*. Embryonic endothelial evolution towards first hematopoietic stem cells revealed by single-cell transcriptomic and functional analyses. Cell Research, 30: 376-392 (2020) (*: Co-corresponding authors).
8. Zhang XM, Wu K, Zheng Y, Zhao H, Gao J, Hou Z, Zhang M, Liao J, Zhang J, Gao Y, Li Y, Li L, Tang Fuchou*, Chen ZJ*, Li J*. In vitro expansion of human sperm through nuclear transfer. Cell Research, 30: 356-359 (2020) (*: Co-corresponding authors).
9. Chen Y, Lyu R, Rong B, Zheng Y, Lin Z, Dai R, Zhang X, Xie N, Wang S, Tang Fuchou*, Lan F*, Tong MH*. Refined spatial temporal epigenomic profiling reveals intrinsic connection between PRDM9-mediated H3K4me3 and the fate of double-stranded breaks. Cell Research, 30: 256-268 (2020) (*: Co-corresponding authors).
10. Wang S, Zheng Y, Li J, Yu Y, Zhang W, Song M, Liu Z, Min Z, Hu H, Jing Y, He X, Sun L, Ma L, Esteban CR, Chan P, Qiao J, Zhou Q, Izpisua Belmonte JC*, Qu J*, Tang Fuchou*, Liu GH*. Single-Cell Transcriptomic Atlas of Primate Ovarian Aging. Cell, 180: 585-600 (2020) (*: Co-corresponding authors).
11. Li J, Wang R, Zhou X, Wang W, Gao S, Mao Y, Wu X, Guo L, Liu H, Wen L, Fu W*, Tang Fuchou*. Genomic and transcriptomic profiling of carcinogenesis in patients with familial adenomatous polyposis. Gut, 69: 1283-1293 (2020) (*: Co-corresponding authors).
12. Wen L, Liu Q, Xu J, Liu X, Shi C, Yang Z, Zhang Y, Xu H, Liu J*, Yang H*, Huang H*, Qiao J*, Tang Fuchou*, Chen ZJ*. Recent advances in mammalian reproductive biology. Science China Life Sciences, 63: 18-58 (2020) (*: Co-corresponding authors).
13. Wen L*, Tang Fuchou*. Human Germline Cell Development: from the Perspective of Single-Cell Sequencing. Molecular Cell, 76: 320-328 (2019) (Review) (*: Co-corresponding authors).
14. Yang X, Hu B, Liao J, Qiao Y, Chen Y, Qian Y, Feng S, Yu F, Dong J, Hou Y, Xu H, Wang R, Peng G*, Li J*, Tang Fuchou*, Jing N*. Distinct enhancer signatures in the mouse gastrula delineate progressive cell fate continuum during embryo development. Cell Research, 29: 911-926 (2019) (*: Co-corresponding authors).
15. Zhou F, Wang R, Yuan P, Ren Y, Mao Y, Li R, Lian Y, Li J, Wen L, Yan L, Qiao J*, Tang Fuchou*. Reconstituting the transcriptome and DNA methylome landscapes of human implantation. Nature, 572: 660-664 (2019) (*: Co-corresponding authors).
16. Soares E, Xu Q, Li Q, Qu J, Zheng Y, Raeven HHM, Brandao KO, Petit I, van den Akker WMR, van Heeringen SJ, Aberdam D, Tang Fuchou*, Zhou H*. Single-cell RNA-seq identifies a reversible mesodermal activation in abnormally specified epithelia of p63 EEC syndrome. Proc Natl Acad Sci U S A, 116: 17361-17370 (2019) (*: Co-corresponding authors).
17. Hu Y, Wang X, Hu B, Mao Y, Chen Y, Yan L, Yong J, Dong J, Wei Y, Wang W, Wen L, Qiao J*, Tang Fuchou*. Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis. PLoS Biology, 17: e3000365 (2019) (*: Co-corresponding authors).
18. Fu L, Hu Y, Song M, Liu Z, Zhang W, Yu FX, Wu J, Wang S, Izpisua Belmonte JC, Chan P, Qu J*, Tang Fuchou*, Liu GH*. Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis. PLoS Biology, 17: e3000201 (2019) (*: Co-corresponding authors).
19. Ren X, Hu B, Song M, Ding Z, Dang Y, Liu Z, Zhang W, Ji Q, Ren R, Ding J, Chan P, Jiang C, Ye K, Qu J*, Tang Fuchou*, Liu GH*. Maintenance of Nucleolar Homeostasis by CBX4 Alleviates Senescence and Osteoarthritis. Cell Reports, 26: 3643-3656 (2019) (*: Co-corresponding authors).
20. Cui Y, Zheng Y, Liu X, Yan L, Fan X, Yong J, Hu Y, Dong J, Li Q, Wu X, Gao S, Li J, Wen L, Qiao J*, Tang Fuchou*. Single-cell transcriptome analysis maps the developmental track of the human heart. Cell Reports, 26: 1934–1950 (2019) (*: Co-corresponding authors).
21. Yan P, Li Q, Wang L, Lu P, Suzuki K, Liu Z, Lei J, Li W, He X, Wang S, Ding J, Chan P, Zhang W, Song M, Izpisua Belmonte JC, Qu J*, Tang Fuchou*, Liu GH*. FOXO3-engineered human ESC-derived vascular cells promote vascular protection and regeneration. Cell Stem Cell, 24: 447-461 (2019) (*: Co-corresponding authors).
22. Ji Q, Zheng Y, Zhang G, Hu Y, Fan X, Hou Y, Wen L, Li L, Xu Y, Wang Y*, Tang Fuchou*. Single-cell RNA-seq analysis reveals the progression of human osteoarthritis. Annals of the Rheumatic Diseases, 78: 100-110 (2019) (*: Co-corresponding authors).
23. Bian S, Hou Y, Zhou X, Li X, Yong J, Wang Y, Wang W, Yan J, Hu B, Guo H, Wang J, Gao S, Mao Y, Dong J, Zhu P, Xiu D, Yan L, Wen L, Qiao J*, Tang Fuchou*, Fu W*. Single-cell multiomics sequencing and analyses of human colorectal cancer. Science, 362: 1060-1063 (2018) (*: Co-corresponding authors).
24. Wang P, Chen Y, Yong J, Cui Y, Wang R, Wen L, Qiao J*, Tang Fuchou*. Dissecting the global dynamic molecular profiles of human fetal kidney development by single-cell RNA sequencing. Cell Reports, 24: 3554-3567 (2018) (*: Co-corresponding authors).
25. Wang M, Liu X, Chang G, Chen Y, An G, Yan L, Gao S, Xu Y, Cui Y, Dong J, Chen Y, Fan X, Hu Y, Song K, Zhu X, Gao Y, Yao Z, Bian S, Hou Y, Lu J, Wang R, Fan Y, Lian Y, Tang W, Wang Y, Liu J, Zhao L, Wang L, Liu Z, Yuan R, Shi Y, Hu B, Ren X, Tang Fuchou*, Zhao XY*, Qiao J*. Single-cell RNA sequencing analysis reveals sequential cell fate transition during human spermatogenesis. Cell Stem Cell, 23: 599-614 (2018) (*: Co-corresponding authors).
26. Chen Y, Zheng Y, Gao Y, Lin Z, Yang S, Wang T, Wang Q, Xie N, Hua R, Liu M, Sha J, Griswold MD, Li J*, Tang Fuchou*, Tong MH*. Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis. Cell Research, 28: 879–896 (2018) (Cover story) (*: Co-corresponding authors).
27. Li L, Guo F, Gao Y, Ren Y, Yuan P, Yan L, Li R, Liang Y, Li J, Hu B, Gao J, Wen L, Tang Fuchou*, Qiao J*. Single-cell multi-omics sequencing of human early embryos. Nature Cell Biology, 20: 847-858 (2018) (*: Co-corresponding authors).
28. Fan X, Dong J, Zhong S, Wei Y, Wu Q, Yan L, Yong J, Sun L, Wang X, Zhao Y, Wang W, Yan J, Wang X*, Qiao J*, Tang Fuchou*. Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis. Cell Research, 28: 730-745 (2018) (*: Co-corresponding authors).
29. Gao S, Yan L, Wang R, Li J, Yong J, Zhou X, Wei Y, Wu X, Wang X, Fan X, Yan J, Zhi X, Gao Y, Guo H, Jin X, Wang W, Mao Y, Wang F, Wen L, Fu W, Ge H*, Qiao J*, Tang Fuchou*. Tracing the temporal-spatial transcriptome landscapes of the human fetal digestive tract using single-cell RNA-sequencing. Nature Cell Biology, 20: 721-734 (2018) (*: Co-corresponding authors).
30. Zhong S, Zhang S, Fan X, Wu Q, Yan L, Dong J, Zhang H, Li L, Sun L, Pan N, Xu X, Tang Fuchou*, Zhang J*, Qiao J*, Wang X*. A single-cell RNA-seq survey of the developmental landscape of the human prefrontal cortex. Nature, 555: 524-528 (2018) (*: Co-corresponding authors).
31. Zhu P, Guo H, Ren Y, Hou Y, Dong J, Li R, Lian Y, Fan X, Hu B, Gao Y, Wang X, Wei Y, Liu P, Yan J, Ren X, Yuan P, Yuan Y, Yan Z, Wen L, Yan L*, Qiao J*, Tang Fuchou*. Single-cell DNA methylome sequencing of human preimplantation embryos. Nature Genetics, 50: 12-19 (2018) (*: Co-corresponding authors).
32. Wen L*, Tang Fuchou*. Boosting the power of single-cell analysis. Nature Biotechnology, 36: 408-409 (2018) (Preview) (*: Co-corresponding authors).
33. Dong J, Hu Y, Fan X, Wu X, Mao Y, Hu B, Guo H, Wen L, Tang Fuchou*. Single-cell RNA-seq analysis unveils a prevalent epithelial/mesenchymal hybrid state during mouse organogenesis. Genome Biology, 19: 31 (2018) (*: Corresponding author).
34. Yang X, Hu B, Hou Y, Qiao Y*, Wang R, Chen Y, Qian Y, Feng S, Chen J, Liu C, Peng G, Tang Fuchou*, Jing N*. Silencing of developmental genes by H3K27me3 and DNA methylation reflects the discrepant plasticity of embryonic and extraembryonic lineages. Cell Research, 28: 593-596 (2018) (*: Co-corresponding authors).
35. Wang S, Hu B, Ding Z, Dang Y, Wu J, Li D, Liu X, Xiao B, Zhang W, Ren R, Lei J, Hu H, Chen C, Chan P, Li D, Qu J*, Tang Fuchou*, Liu GH*. ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells. Cell Discovery, 4: 2 (2018) (*: Co-corresponding authors).
36. Wen L* & Tang Fuchou*. Single cell epigenome sequencing technologies. Molecular Aspects of Medicine, 59: 62-69 (2018) (Review) (*: Co-corresponding authors).
37. Liu J, Liu W, Yang L, Wu Q, Zhang H, Fang A, Li L, Xu X, Sun L, Zhang J*, Tang Fuchou*, Wang X*. The primate-specific gene TMEM14B marks outer radial glia cells and promotes cortical expansion and folding. Cell Stem Cell, 21: 635-649 (2017) (*: Co-corresponding authors).
38. Guo F*, Li L, Li J, Wu X, Hu B, Zhu P, Wen L, Tang Fuchou*. Single-cell multi-omics sequencing of mouse early embryos and embryonic stem cells. Cell Research, 27: 967-988 (2017) (Cover story) (*: Co-corresponding authors).
39. Yang J, Li J, Suzuki K, Liu X, Wu J, Zhang W, Ren R, Zhang W, Chan P, Izpisua Belmonte JC, Qu J*, Tang Fuchou*, Liu GH*. Genetic enhancement in cultured human adult stem cells conferred by a single nucleotide recoding. Cell Research, 27: 1178-1181 (2017) (*: Co-corresponding authors).
40. Li L, Dong J, Yan L, Yong J, Liu X, Hu Y, Fan X, Wu X, Guo H, Wang X, Zhu X, Li R, Yan J, Wei Y, Zhao Y, Wang W, Ren Y, Yuan P, Yan Z, Hu B, Guo F, Wen L, Tang Fuchou*, Qiao J*. Single-cell RNA-seq analysis maps development of human germline cells and gonadal niche interactions. Cell Stem Cell, 20: 858-873 (2017) (*: Co-corresponding authors).
41. Zhu C, Gao Y, Guo H, Xia B, Song J, Wu X, Zeng H, Kee K, Tang Fuchou*, Yi C*. Single-cell 5-formylcytosine landscapes of mammalian early embryos and ESCs at single-base resolution. Cell Stem Cell, 20: 720-731 (2017) (*: Co-corresponding authors).
42. Guo H, Hu B, Yan L, Yong J, Wu Y, Gao Y, Guo F, Hou Y, Fan X, Dong J, Wang X, Zhu X, Yan J, Wei Y, Jin H, Zhang W, Wen L, Tang Fuchou*, Qiao J*. DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells. Cell Research, 27: 165-183 (2017) (*: Co-corresponding authors).
43. Yang L, Ma Z, Cao C, Zhang Y, Wu X, Lee R, Hu B, Wen L, Ge H, Huang Y*, Lao K*, Tang Fuchou*. MR-seq: measuring a single cell’s transcriptome repeatedly by RNA-seq. Science Bulletin, 62: 391-398 (2017) (*: Co-corresponding authors).
44. Zhou F, Li X, Wang W, Zhu P, Zhou J, He W, Ding M, Xiong F, Zheng X, Li Z, Ni Y, Mu X, Wen L, Cheng T, Lan Y, Yuan W*, Tang Fuchou*, Liu B*. Tracing haematopoietic stem cell formation at single-cell resolution. Nature, 533: 487-492 (2016) (*: Co-corresponding authors).
45. Wen L*, Tang Fuchou*. Single-cell sequencing in stem cell biology. Genome Biology 17: 71 (2016) (Review) (*: Co-corresponding authors).
46. Dang Y, Yan L, Hu B, Fan X, Ren Y, Li R, Lian Y, Yan J, Li Q, Zhang Y, Li M, Ren X, Huang J, Wu Y, Liu P, Wen L, Zhang C, Huang Y*, Tang Fuchou*, Qiao J*. Tracing the expression of circular RNAs in human pre-implantation embryos. Genome Biology 17: 130 (2016) (*: Co-corresponding authors).
47. Hou Y, Guo H, Cao C, Li X, Hu B, Zhu P, Wu X, Wen L, Tang Fuchou*, Huang Y*, Peng J*. Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas. Cell Research 26: 304-319 (2016) (*: Co-corresponding authors).
48. Pan H, Guan D, Liu X, Li J, Wang L, Wu J, Zhou J, Zhang W, Ren R, Zhang W, Li Y, Yang J, Hao Y, Yuan T, Yuan G, Wang H, Ju Z, Mao Z, Li J, Qu J*, Tang Fuchou*, Liu GH*. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Research 26: 190-205 (2016) (*: Co-corresponding authors).
49. Yan L, Guo H, Hu B, Li R, Yong J, Zhao Y, Zhi X, Fan X, Guo F, Wang X, Wang W, Wei Y, Wang Y, Wen L, Qiao J*, Tang Fuchou*. Epigenomic landscape of human fetal brain, heart, and liver. Journal Biological Chemistry 291: 4386-4398 (2016) (*: Co-corresponding authors).
50. Yan L, Huang L, Xu L, Huang J, Ma F, Zhu X, Tang Y, Liu M, Lian Y, Liu P, Li R, Lu S, Tang Fuchou*, Qiao J*, Xie XS*. Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses. Proc Natl Acad Sci U S A 112: 15964-15969 (2015) (*: Co-corresponding authors).
51. Wen L*, Tang Fuchou*, How to catch rare cell types. Nature 52: 197-198 (2015) (*: Co-corresponding authors) (Preview).
52. Guo F, Yan L, Guo H, Li L, Hu B, Zhao Y, Yong J, Hu Y, Wang X, Wei Y, Wang W, Li R, Yan J, Zhi X, Zhang Y, Jin H, Zhang W, Hou Y, Zhu P, Li J, Zhang L, Liu S, Ren Y, Zhu X, Wen L, Gao Y, Tang Fuchou*, Qiao J*. The transcriptome and DNA methylome landscapes of human primordial germ cells. Cell 161: 1437-1452 (2015) (*: Co-corresponding authors).
53. Zhang W, Li J, Suzuki K, Qu J, Wang P, Zhou J, Liu X, Ren R, Xu X, Ocampo A, Yuan T, Yang J, Li Y, Shi L, Guan D, Pan H, Suan S, Ding Z, Li M, Yi F, Bai R, Wang Y, Chen C, Yang F, Li X, Wang Z, Aizawa E, Goebl A, Soligalla RE, Reddy P, Esteban CR, Tang Fuchou*, Liu G* and Izpisua Belmonte JC*. A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging. Science 348: 1160-1163 (2015) (*: Co-corresponding authors).
54. Wen L*, Tang Fuchou*. Charting a map through the cellular reprogramming landscape. Cell Stem Cell 16: 215-216 (2015) (*: Co-corresponding authors) (Preview).
55. Wen L, Li J, Guo H, Liu X, Zheng S, Zhang D, Zhu W, Qu J, Guo L, Du D, Jin X, Zhang Y, Gao Y, Shen J, Ge H, Tang Fuchou*, Huang Y*, Peng J*. Genome-scale detection of hypermethylated CpG islands in circulating cell-free DNA of hepatocellular carcinoma patients. Cell Research 25: 1250-1264 (2015) (*: Co-corresponding authors).
56. Duan S, Yuan G, Liu X, Ren R, Li J, Zhang W, Wu J, Xu X, Fu L, Li Y, Yang J, Zhang W, Bai R, Yi F, Suzuki K, Gao H, Esteban CR, Zhang C, Izpisua Belmonte JC, Chen Z, Wang X, Jiang T, Qu J*, Tang Fuchou*, Liu GH*. PTEN deficiency reprogrammes human neural stem cells towards a glioblastoma stem cell-like phenotype. Nature Communications 6: 10068 (2015) (*: Co-corresponding authors).
57. Fan X, Zhang X, Wu X, Guo H, Hu Y, Tang Fuchou*, Huang Y*. Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos. Genome Biology 16: 148 (2015) (*: Co-corresponding authors).
58. Guo H, Zhu P, Guo F, Li X, Wu X, Fan X, Wen L*, Tang Fuchou*. Profiling DNA methylome landscapes of mammalian cells with single-cell reduced-representation bisulfite sequencing. Nature Protocols 10: 645-659 (2015) (*: Co-corresponding authors).
59. Wu Y, Zhou H, Fan X, Zhang Y, Zhang M, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L* , Tang Fuchou*, Li J,* Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Cell Research 25: 67-79 (2015) (*: Co-corresponding authors).
60. Shen J, Jiang D, Fu Y, Wu X, Guo H, Feng B, Pang Y, Streets AM, Tang Fuchou*, Huang Y*. H3K4me3 epigenomic landscape derived from ChIP-Seq of 1 000 mouse early embryonic cells. Cell Research 25: 143-147 (2015) (*: Co-corresponding authors).
61. Guo F, Li X, Liang D, Li T, Zhu P, Guo H, Wu X, Wen L, Gu TP, Hu B, Walsh CP, Li J*, Tang Fuchou*, Xu GL*. Active and passive demethylation of male and female pronuclear dna in the mammalian zygote. Cell Stem Cell 15: 447-458 (2014) (*: Co-corresponding authors).
62. Guo H, Zhu P, Yan L, Li R, Hu B, Lian Y, Yan J, Ren X, Lin S, Li J, Jin X, Shi X, Liu P, Wang X, Wang W, Wei Y, Li X, Guo F, Wu X, Fan X, Yong J, Wen L, Xie SX, Tang Fuchou*, Qiao J*. The DNA methylation landscape of human early embryos. Nature 511: 606-610 (2014) (*: Co-corresponding authors).
63. Wen L, Tang Fuchou*. Reconstructing complex tissues from single-cell analyses. Cell 157: 771-773 (2014) (*: Corresponding author) (Preview).
64. Streets AM, Zhang X, Cao C, Pang Y, Wu X, Xiong L, Yang L, Fu Y, Zhao L*, Tang Fuchou*, Huang Y*. Microfluidic single-cell whole-transcriptome sequencing. Proc Natl Acad Sci U S A 111: 7048-7053 (2014) (*: Co-corresponding authors).
65. Wen L, Li X, Yan L, Tan Y, Li R, Zhao Y, Wang Y, Xie J, Zhang Y, Song C, Yu M, Liu X, Zhu P, Li X, Hou Y, Guo H, Wu X, He C*, Li R*, Tang Fuchou*, Qiao J*. Whole-genome analysis of 5-hydroxymethylcytosine and 5-methylcytosine at base resolution in the human brain. Genome Biology 15: R49 (2014) (*: Co-corresponding authors).
66. Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, Li J, Xu L, Tang Fuchou*, Xie XS*, Qiao J*. Genome analyses of single human oocytes. Cell 155:1492-1506 (2013) (*: Co-corresponding authors).
67. Guo H, Zhu P, Wu X, Li X, Wen L, Tang Fuchou*. Single-cell methylome landscapes of mouse embryonic stem cells and early embryos analyzed using reduced representation bisulfite sequencing. Genome Research 23: 2126-2135 (2013) (*: Corresponding author).
68. Yan L, Yang M, Guo H, Yang L, Wu J, Li R, Liu P, Lian Y, Zheng X, Yan J, Huang J, Li M, Wu X, Wen L, Lao K, Li R*, Qiao J*, Tang Fuchou*. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells. Nature Structural & Molecular Biology 20: 1131-1139 (2013) (*: Co-corresponding authors).
69. Gan H, Wen L, Liao S, Lin X, Ma T, Liu J, Song CX, Wang M, He C, Han C*, Tang Fuchou*. Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis. Nature Communications 4: 1995 (2013) (*: Co-corresponding authors).
70. Tang Fuchou*, Barbacioru C*, Nordman E, Bao S, Lee C, Wang X, Tuch BB, Heard E, Lao K, Surani MA. Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One 6: e21208 (2011) (*: Co-first authors).
71. Tang Fuchou, Lao K, Surani MA. Development and applications of single-cell transcriptome analysis. Nature Methods 8: S6-S11 (2011) (Review).
72. Tang Fuchou, Barbacioru C, Bao S, Lee C, Nordman E, Wang X, Lao K, Surani MA. Tracing the derivation of embryonic stem cells from the inner cell mass by single-cell RNA-Seq analysis. Cell Stem Cell 6: 468 - 478 (2010).
73. Tang Fuchou*. Small RNAs in mammalian germline: tiny for immortal. Differentiation 79: 141-146 (2010) (*: Corresponding author) (Review).
74. Tang Fuchou, Barbacioru C, Nordman E, Li B, Xu N, Bashkirov VI, Lao K, Surani MA. RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nature Protocols 5: 516-535 (2010).
75. Bao S*, Tang Fuchou*, Li X, Hayashi K, Gillich A, Lao K, Surani MA. Epigenetic reversion of postimplantation epiblast to pluripotent embryonic stem cells. Nature 461: 1292 - 1295 (2009) (*: Co-first authors).
76. Tang Fuchou*, Barbacioru C*, Wang Y, Nordman E, Lee C, Xu N, Wang X, Bodeau J, Tuch BB, Siddiqui A, Lao K, Surani MA. mRNA-Seq whole transcriptome analysis of a single cell. Nature Methods 6: 377 - 382 (2009) (*: Co-first authors).
77. Tang Fuchou, Hajkova P, O`Carroll D, Lee C, Tarakhovsky A, Lao K, Surani MA. MicroRNAs are tightly associated with RNA-induced gene silencing complexes in vivo. Biochemical & Biophysical Research Communications 372: 24 - 29 (2008).
78. Tang Fuchou, Hayashi K, Kaneda M, Lao K, Surani MA. A sensitive multiplex assay for piRNA expression. Biochemical & Biophysical Research Communications 369: 1190 - 1194 (2008).
79. Tang Fuchou*, Kaneda M*, O`Carroll D, Hajkova P, Barton SC, Sun YA, Lee C, Tarakhovsky A, Lao K, Surani MA. Maternal microRNAs are essential for mouse zygotic development. Genes & Development 21: 644 - 648 (2007) (*: Co-first authors).
80. Tang Fuchou, Hajkova P, Barton SC, O`Carroll D, Lee C, Lao K, Surani MA. 220-plex microRNA expression profile of a single cell. Nature Protocols 1: 1154 - 1159 (2006).
81. Tang Fuchou, Hajkova P, Barton SC, Lao K, Surani MA. MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Research 34: e9 (2006).